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| Nogueira,AHC; Silva,C; Gomes,DE; Rosa,ACG; Luvizotto,MCR; Cardoso,TC. |
| Rabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two "in-house" enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies antibody; Bayesian analysis; ELISA assay. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100012 |
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| Luvizotto,MCR; Carreira,VS; Ferrari,HF; Ribeiro,D; Vallim,MA; Azevedo,V; Cardoso,TC. |
| A four-year-old male goat with a history of neurological disorder was euthanized. It presented uncommon nodules in the brain and lungs associated with multiple abscesses, predominantly in the spleen and liver. Histological examination of brain and lung sections revealed yeast forms confirmed to be Cryptococcus gattii after a combination of isolation and polymerase chain reaction (PCR) procedures. Moreover, Corynebacterium pseudotuberculosis infection was diagnosed by PCR of samples from the lung, spleen and liver. The present report highlights the rare concurrent infection of C. gatti and C. pseudotuberculosis in an adult goat from São Paulo state, Brazil, and indicates the necessity of surveillance in the treatment of goats with atypical pulmonary... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus gattii; Corynebacterium pseudotuberculosis; Meningoencephalitis; Goat. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300015 |
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| Cardoso,TC; Oliveira,C; Da-Silva,SEL; Ferreira,HL; Pinto,AA. |
| Broilers were spray-vaccinated (n=150) with H120 serotype at one-day-old, challenged after 28 days with M41 IBV serotype and after bled at day 28, 34 and 46 after challenged. The respective sera were tested by the indirect ELISA (I-ELISA), sandwich ELISA (S-ELISA), liquid phase blocking ELISA (LPB-ELISA) and the standard serum neutralization test (SNT). For this purpose, a total of 300 sera samples, 150 from non vaccinated and 150 from vaccinated broilers were titrated by all the serological methods and the correlation coefficients were determined. The correlation values (r) between LPB-ELISA x SNT, S-ELISA x SNT, I-ELISA x SNT found werer = 0.98, r = 0.79, and r = 0.74, respectively. Nevertheless, the r between LPB-ELISA x S-ELISA, LPB-ELISA x I-ELISA and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Post vaccinal response; Infectious bronchitis virus; Broilers. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000100010 |
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| Luvizotto,MCR; Ferrari,HF; Cardoso,TC. |
| Infection of susceptible ruminants, including domestic cattle (Bos taurus) and American bison (Bison bison), with ovine herpesvirus-2 (OvHV-2) may provoke the fatal vasculitis and lymphoproliferative syndrome, known as malignant catarrhal fever (MCF), reported worldwide. To the best of our knowledge, this is the first report of a clinical case of MCF-like lesions associated with ovine herpesvirus-2 (OvHV-2) infection in young calves (Bos indicus) including central nervous symptoms that occurred in Três Lagoas city, Mato Grosso do Sul state, a border town near São Paulo state, Brazil. The diagnosis was based on typical histological lesions characterized by systemic lymphohistiocytic and fibrinoid vasculitis, confirmed by polymerase chain reaction and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovine herpesvirus-2; Malignant catarrhal fever (MCF); Meningoencephalitis; Cattle. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100018 |
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| Curci,VCM; Nogueira,AHC; Nobrega,FLC; Araujo,RF; Perri,SHV; Cardoso,TC; Dutra,IS. |
| Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostridium botulinum types C and D; vaccines 1 and 2) and two polyvalent (all Clostridium spp. including Clostridium botulinum types C and D; vaccines 3 and 4), that are currently used in Brazilian herds, were tested in order to verify the maternal immune response. One hundred cows, divided into four vaccinated groups and one unvaccinated group, were given a two-dose subcutaneous immunization, at day zero,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Clostridium botulinum; Neonatal immunity; Cattle; Botulism; Brazil. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300018 |
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| Silva,SEL; Ferreira,HL; Carvalho,BA; Cardoso,TC. |
| Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avibirnavirus; CER cells; Diagnosis; Double antibody sandwich ELISA; RT/PCR. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100009 |
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